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Merck
CN

SAB4200601

Monoclonal Anti-SLC5A7 antibody produced in mouse

~1.0 mg/mL, clone 62-2E8, purified immunoglobulin

Synonym(s):

Anti-MGC126299, Anti-MGC126300, Anti-SLC5A7, Anti-carrier family 5 (choline transporter), member 7, Anti-CHT, Anti-CHT1, Anti-Choline transporter, Anti-Hact, Anti-hCHT

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
62-2E8, monoclonal
Species reactivity:
mouse, canine, human, bovine, monkey, chicken
Application:
ICC, IF
Citations:
1
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biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

62-2E8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~72 kDa

species reactivity

mouse, canine, human, bovine, monkey, chicken

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using SK-Mel-28 total cell extracts, immunocytochemistry: suitable, immunofluorescence: 5-10 μg/mL using HEK-293T cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KHSRP(8570)

General description

Monoclonal Anti-SLC5A7 (mouse IgG1 isotype) is derived from the hybridoma 62-2E8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human SLC5A7 recombinant fusion protein.

Immunogen

human SLC5A7 recombinant fusion protein

Application

Monoclonal Anti-SLC5A7 antibody produced in mouse may be used in:
  • immunoblotting
  • immunofluorescence
  • immunocytochemistry

Biochem/physiol Actions

Monoclonal Anti- SLC5A7 recognizes human, bovine, monkey, chicken, dog and mouse SLC5A7.
Solute carrier family 5 (sodium/choline cotransporter), member 7 (SLC5A7) is responsible for the reuptake of choline, the rate-limiting step in acetylcholine synthesis from the synaptic cleft into presynaptic neurons. A significant dysfunction of cholinergic neurotransmission is observed in a number of neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Related Content


The ?ins? and ?outs? of the high-affinity choline transporter CHT1
Ribeiro FM, et al.
Journal of Neurochemistry, 97(1), 1-12 (2006)



Global Trade Item Number

SKUGTIN
SAB4200601-200UL04061838037091