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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
62-2E8, monoclonal
Species reactivity:
mouse, canine, human, bovine, monkey, chicken
Application:
ICC, IF
Citations:
1
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
62-2E8, monoclonal
form
buffered aqueous solution
mol wt
antigen ~72 kDa
species reactivity
mouse, canine, human, bovine, monkey, chicken
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using SK-Mel-28 total cell extracts, immunocytochemistry: suitable, immunofluorescence: 5-10 μg/mL using HEK-293T cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KHSRP(8570)
General description
Monoclonal Anti-SLC5A7 (mouse IgG1 isotype) is derived from the hybridoma 62-2E8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human SLC5A7 recombinant fusion protein.
Immunogen
human SLC5A7 recombinant fusion protein
Application
Monoclonal Anti-SLC5A7 antibody produced in mouse may be used in:
- immunoblotting
- immunofluorescence
- immunocytochemistry
Biochem/physiol Actions
Monoclonal Anti- SLC5A7 recognizes human, bovine, monkey, chicken, dog and mouse SLC5A7.
Solute carrier family 5 (sodium/choline cotransporter), member 7 (SLC5A7) is responsible for the reuptake of choline, the rate-limiting step in acetylcholine synthesis from the synaptic cleft into presynaptic neurons. A significant dysfunction of cholinergic neurotransmission is observed in a number of neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Related Content
Instructions
The ?ins? and ?outs? of the high-affinity choline transporter CHT1
Ribeiro FM, et al.
Journal of Neurochemistry, 97(1), 1-12 (2006)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAB4200601-200UL | 04061838037091 |