SAB4200681
Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal
clone GFP-20, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse, green fluorescent protein
Sign In to View Organizational & Contract Pricing
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
GFP-20, monoclonal
form
buffered aqueous solution
mol wt
antigen 27 kDa
species reactivity
rat, human
concentration
~1 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein.
immunoprecipitation (IP): 5-10 μg using whole extract of human HEK-293T cells over-expressing GFP tagged fusion protein.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal anti-green fluorescent protein (GFP) (mouse IgG1 isotype) is derived from the GFP-20 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse BALB/c mice immunized with a GFP tagged fusion protein.GFP is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+-activated photoprotein aequorin to GFP.
Immunogen
GFP tagged fusion protein
Application
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse may be used in:
- immunoblotting
- immunoprecipitation
- dot blot
- enzyme-linked immunosorbent assay (ELISA)
- scanning electron microscope (SEM)
Biochem/physiol Actions
GFP (green fluorescence protein) is a reporter molecule which is used for checking gene expression and protein localization. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence.
Monoclonal Anti-Green Fluorescent Protein (GFP) recognizes N-terminal and C-terminal GFP (27 kDa) tagged fusion proteins. The antibody reacts with fusion proteins expressed by prokaryotes expression vectors.
Physical form
a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Preparation Note
For continuous use, store at 28 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The Green Fluorescent Protein
Tsien RY
Annual Review of Biochemistry, 67, 509-544 (1998)
Expression and Detection of Green Fluorescent Protein (GFP)
Kain SR and Kitts P.
Methods in Molecular Biology, 63, 305-324 (1997)
Ilenia Meloni et al.
Scientific reports, 10(1), 17614-17614 (2020-10-21)
Invertebrates such as Drosophila melanogaster have proven to be a valuable model organism for studies of the nervous system. In order to control neuronal activity, optogenetics has evolved as a powerful technique enabling non-invasive stimulation using light. This requires light
Green Fluorescent Protein as a Reporter for Macromolecular Localization in Bacterial Cells
Margolin W
Methods, 20, 62-72 (2000)
Sven Dannhäuser et al.
eLife, 9 (2020-10-01)
Adhesion-type GPCRs (aGPCRs) participate in a vast range of physiological processes. Their frequent association with mechanosensitive functions suggests that processing of mechanical stimuli may be a common feature of this receptor family. Previously, we reported that the Drosophila aGPCR CIRL
Related Content
Instructions
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service