SAB4200686
Anti-Collagen, Type VII antibody, Mouse monoclonal
clone LH7.2, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Collagen, Type VII antibody produced in mouse, COL7A1, EBD1, EBDCT, EBR1, alpha 1, collagen, type VII
Sign In to View Organizational & Contract Pricing
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LH7.2, monoclonal
form
buffered aqueous solution
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoblotting: suitable
immunohistochemistry: 1.5-3 μg/mL using frozen human tonsil sections
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... COL7A1(1294)
Related Categories
General description
Monoclonal Anti-Collagen Type VII (mouse IgG1 isotype) is derived from the hybridoma LH7.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Collagen Type VII (known also as Col7), which belongs to the collagen superfamily, is a major extracellular matrix component of the anchoring fibrils in lamina densa. COL7A1 (collagen type VII alpha 1 chain) gene codes for type VII collagen. It is located on human chromosome 3p. It is expressed by keratinocytes and fibroblasts. COL7A1 is the important component the anchoring fibrils.
Immunogen
insoluble fractions prepared from neonatal foreskin epidermal cells
Application
Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunohistochemistry
- immunofluorescence
Biochem/physiol Actions
Collagen type VII is very essential for adhesive connection between the dermis and the epidermal basement membrane zone (BMZ). Mutations in collagen type VII cause dystrophic forms of epidermolysis bullosa (including recessive dystrophic epidermolysis bullosa (RDEB)), which manifest as skin fragility and malformed enamel.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
常规特殊物品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Julia Niskanen et al.
PloS one, 12(5), e0177527-e0177527 (2017-05-12)
A rare hereditary mechanobullous disorder called epidermolysis bullosa (EB) causes blistering in the skin and the mucosal membranes. To date, nineteen EB-related genes have been discovered in human and other species. We describe here a novel EB variant in dogs.
Nonsense variant in COL7A1 causes recessive dystrophic epidermolysis bullosa in Central Asian Shepherd dogs
Niskanen J, et al.
PLoS ONE, 12(5), e0177527-e0177527 (2017)
Genetic linkage of type VII collagen (COL7A1) to dominant dystrophic epidermolysis bullosa in families with abnormal anchoring fibrils.
Ryynaanen M, et al.
The Journal of Clinical Investigation (1992)
Deletion of a Pathogenic Mutation-Containing Exon of COL7A1 Allows Clonal Gene Editing Correction of RDEB Patient Epidermal Stem Cells.
Mencia A, et al.
Molecular Therapy. Nucleic Acids, 11, 68-78 (2018)
Dystrophic epidermolysis bullosa: COL7A1 mutation landscape in a multi-ethnic cohort of 152 extended families with high degree of customary consanguineous marriages.
Vahidnezhad H, et al.
The Journal of Investigative Dermatology, 137(3), 660-669 (2017)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service