SAB4200731
Anti-Pan-Cadherin antibody, Mouse monoclonal
clone CH-19, purified from hybridoma cell culture
Synonym(s):
Anti-Pan Cadherin
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
CH-19, monoclonal
Application:
IF, IHC
Citations:
9
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CH-19, monoclonal
form
buffered aqueous solution
mol wt
~135 kDa
species reactivity
guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 0.125-0.25 μg/mL using HeLa cell extract, immunofluorescence: 5-10 μg/mL using HeLa cells, immunohistochemistry: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CDH1(999)
General description
Anti-Pan-Cadherin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CH-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with the synthetic peptide.
N-cadherin, also known as cadherin-2 (CDH2) or neural cadherin (NCAD) is mapped to human chromosome 18q12.1. N-cadherin has ectodomains comprising of 110 amino acidsand is called the extracellular cadherin (EC) domain. EC domains are critical for the adhesion function of N-cadherin.
Immunogen
synthetic peptide corresponding to the C-terminus of chicken N-Cadherin with an extra N-terminal lysine residue coupled with KLH.
Application
Anti-Pan-Cadherin antibody, Mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Biochem/physiol Actions
Monoclonal Anti-Pan-Cadherin recognizes Cadherin from human, mouse, monkey, bovine, chicken, rat, ferret, canine, rabbit, pig, guinea pig, hamster, cat, frog, snake and psammomys origin. Monoclonal Anti Pan-Cadherin is reactive against a highly conserved sequence at the cytoplasmic C-terminal of N-Cadherin protein.
N-cadherin plays a key role in the neurulation, which involves transition of neural plate into neural tube. It is present in the synaptic membrane and dimerises upon synaptic induction and mediates synaptic activity. N-cadherin mediates cell−cell adhesion in retina but favors tumor progression in retinoblastoma. It is upregulated in carcinomas and is critical for cell invasion. Irregular distribution of N-cadherin is implicated in dilated cardiomyopathy. Mutations in the CHD2 gene may probably contribute to obsessive-compulsive disorder (OCD) and Tourette disorder (TD).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
Structure-function analysis of cell adhesion by neural (N-) cadherin
Tamura K, et al.
Neuron, 20(6), 1153-1163 (1998)
Molecular modification of N-cadherin in response to synaptic activity
Tanaka H, et al.
Neuron, 25(1), 93-107 (2000)
Structure and function of the N-cadherin/catenin complex in retinoblastoma
Van A, et al.
Investigative Ophthalmology & Visual Science, 43(3), 595-602 (2002)
Apoptosis-related factors p53, bcl-2 and the defects of force transmission in dilated cardiomyopathy
Tsipis A , et al.
Pathology Research and Practice, 206(9), 625-630 (2010)
Structure and biochemistry of cadherins and catenins
Shapiro L and Weis WI
Cold Spring Harbor Perspectives in Biology, a003053-a003053 (2009)
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