SAB4200738
Anti-FITC antibody, Mouse monoclonal
clone FL-D6, purified from hybridoma cell culture
Synonym(s):
Anti-Fluorescein isothiocyanate
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About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Clone:
FL-D6, monoclonal
Technique(s):
immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC
Application:
ELISA (i), IHC
Citations:
2
biological source
mouse
Quality Segment
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
FL-D6, monoclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-FITC antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the FL-D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with FITC (fluorescein isothiocyanate)-BSA conjugate. This antibody specifically recognizes both free and conjugated FITC.
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light (with peak wavelength of approximately 495 nm) causing molecules to become excited and emit a visible yellow-green light (with peak wavelength of approximately 519 nm).
Immunogen
FITC-BSA conjugate
Application
Anti-FITC antibody, Mouse monoclonal may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
Biochem/physiol Actions
FITC (fluorescein isothiocyanate) is a commonly used protein marker including antibodies in immunofluorescent techniques. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well-defined hapten.
Monoclonal Anti-FITC antibody specifically recognizes both free and conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Discard working dilution samples if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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