Skip to Content
Merck
CN

SAB4200752

Sigma-Aldrich

Anti-Nuclease P1 antibody produced in rabbit

Ig fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Deoxyribonuclease P1, Anti-Endonuclease P1

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352202
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

antibody form

Ig fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~30 kDa

concentration

~1 mg/mL

technique(s)

immunoblotting: 1:10,000-1:20,000 using Nuclease P1 from Penicillium citrinum

UniProt accession no.

P24289

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Nuclease P1 (Nuclease 5′-Nucleotidehydrolase, 3′- Phosphohydrolase, EC 3.1.30.1), also known as deoxyribonuclease P1 or endonuclease P1, is a zinc dependent endonuclease. It is isolated from Penicillium citrinum.

Immunogen

Nuclease P1 from Penicillium citrinum

Application

Anti-Nuclease P1 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Nuclease P1 catalyzes the hydrolysis of 3′-5′ phosphodiester bonds in RNA and single-stranded DNA and 3′-phosphomonoester bonds in mono- and oligonucleotides terminated by a 3′-phosphate group without apparent bases specificity. The enzyme is used as a component of nuclease cocktail to generate single-stranded oligodeoxynucleotide (ODNs) samples for mass spectroscopy research for characterization of oligodeoxyribonucleotides. Nuclease P1 has also been widely used in the food industry to enhance or create flavor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
037M4763V
51170201

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Identity of Phosphodiesterase and Phosphomonoesterase Activities with Nuclease P1 (a Nuclease from Penicillium citrinum)
Fujimoto, M., et al.
Agricultural and Biological Chemistry, 38, 785-790 (1974)
Production, purification and characterization of nuclease p1 from Penicillium citrinum
Guo-Qing Y, et al.
Process Biochemistry (Oxford, United Kingdom), 41(6), 1276-1281 (2006)
Nuclease digestion and mass spectrometric characterization of oligodeoxyribonucleotides containing 1, 2-GpG, 1, 2-ApG, and 1, 3-GpXpG cisplatin intrastrand cross-links
Williams RT, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 420(6), 160-170 (2013)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service