SAB4200771
Anti-Surfactant Protein D antibody, Mouse monoclonal
clone 10F6E12, purified from hybridoma cell culture
Synonym(s):
Anti-Collectin-7, Anti-Lung surfactant protein D, Anti-PSP-D, Anti-Pulmonary surfactant-associated protein D, Anti-SP-D
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About This Item
UNSPSC Code:
12352200
Clone:
10F6E12, monoclonal
Species reactivity:
mouse, human
Application:
—
Citations:
10
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
10F6E12, monoclonal
form
buffered aqueous solution
mol wt
~43 kDa
species reactivity
mouse, human
concentration
~1 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using mouse lung extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SFTPD(6441)
mouse ... Sftpd(20390)
General description
Surfactant Protein D (SFTPD) belongs to the collectin family of multimeric glycoproteins and exists as oligomeric structure or a trimeric subunit. The human SFTPD corresponds to a molecular weight of 150kDa and the trimer is close to 620 kDa. Anti-Surfactant Protein D antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the 10F6E12 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with mouse recombinant SP-D.
Surfactant Protein D (SP-D) contains an N-terminal cysteine-rich domain, a collagenous domain, a neck region and a C-terminal carbohydrate recognition domain (CRD). The gene is located on human chromosome 14. SP-D is expressed in lungs and kidney.
Immunogen
Mouse recombinant SP-D
Application
Anti-Surfactant Protein D antibody, Mouse monoclonal may be used in immunoblotting.
Biochem/physiol Actions
Anti-Surfactant Protein D antibody, Mouse monoclonal recognizes SP-D from human and mouse origin.
Surfactant Protein D (SFTPD) imparts pulmonary defense during fungal infection. It favors pulmonary inflammation. It recognizes the surface lipopolysaccharide (LPS) of pathogens and acts as first-line host defense molecule. It also recognizes the viral glycoprotein hemagglutinin (HA) present in viral surface. Polymorphism in the SFTPD gene may lead to decreased levels of the protein and high risk for chronic obstructive pulmonary disease (COPD).
Surfactant Protein D (SP-D) plays a critical role in the innate host defense and the modulation of inflammatory responses in infectious diseases. It aids the uptake and clearance of pathogens and apoptotic cells. SP-D maintains surfactant homeostasis. It reduces the surface tension at the air-liquid interface and blocks the lung from collapsing.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
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Crystal structure of a complex of surfactant protein D (SP-D) and Haemophilus influenzae lipopolysaccharide reveals shielding of core structures in SP-D-resistant strains
Clark HW, et al.
Infection and Immunity, 84(5), 1585-1592 (2016)
Purification, characterization and cDNA cloning of human lung surfactant protein D
Lu J, et al.
The Biochemical Journal, 284(3), 795-802 (1992)
Single nucleotide polymorphisms in collagenous lectins and other innate immune genes in pigs with common infectious diseases
Keirstead ND, et al.
Veterinary Immunology and Immunopathology, 142(1-2), 1-13 (2011)
Molecular mechanisms of inhibition of influenza by surfactant protein D revealed by large-scale molecular dynamics simulation
Goh BC, et al.
Biochemistry, 52(47), 8527-8538 (2013)
Genetic loci associated with chronic obstructive pulmonary disease overlap with loci for lung function and pulmonary fibrosis
Hobbs BD, et al.
Nature Genetics, 49(3), 426-426 (2017)
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