Skip to Content
Merck
CN

SAB4200802

Sigma-Aldrich

Anti-Epidermal Growth Factor (EGF) Receptor antibody, Mouse monoclonal

clone F4, purified from hybridoma cell culture

Synonym(s):

Anti-EGFR, Anti-Proto-oncogene c-ErbB-1, Anti-Receptor tyrosine-protein kinase erbB-1

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

F4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~170 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.3-0.6 μg/mL using human epidermoid carcinoma A431 cell line extract
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P00533

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

General description

Epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 or HER-1, is an integral cell membrane protein which spans the membranes of a wide range of normal and malignant epithelial cells. EGFR is a tyrosine-specific protein kinase domain, it has an extracellular region which binds EGF and an intra-cellular carboxy-terminal region which possesses the tyrosine kinase activity.
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the F4 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse.

Immunogen

peptide corresponding to the C-terminal region of human EGF receptor (EGFR) (GeneID 1956), coupled to KLH

Application

Anti-Epidermal Growth Factor (EGF) Receptor antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor recognizes intracellular domain of the EGF receptor. The antibody is recommended to use in various immunological techniques, including Immunobloting (∼170 kDa), Immunoprecipitation, Immunohistochemistry and Immunofluorescence.

Biochem/physiol Actions

Epidermal growth factor (EGF) binding to its specific EGFR, results in an increased DNA synthesis initiating a signaling cascades including cell proliferation, differentiation and repair of damaged epithelial tissue. The EGFR has a half-life of approximately ~10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1hour. A close similarity has been found between the sequence of the v-erb-B oncogene and the cytoplasmic transmembrane domain of the EGFR (truncated EGFR). Overexpression or mutation of EGFR may enhance DNA repair mechanisms, thereby contributing to radio- and chemotherapy resistance. High levels of EGFR have been identified in sarcomas, gliomas, gynecological, breast, bladder and lung tumors.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
058M4821
51180224

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service