SAB4300695
Anti-CDK4 antibody produced in rabbit
affinity isolated antibody
Synonym(s):
Anti-CMM3, Anti-PSK-J3
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~34 kDa
species reactivity
human
concentration
1 mg/mL
technique(s)
western blot: 1:500-1:1000
isotype
IgG
immunogen sequence
(L-H-K-D-E)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDK4(1019)
General description
Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
The antibody detects endogenous levels of total CDK4 protein.
Immunogen
Peptide sequence around aa. 295-299 (L-H-K-D-E), according to the protein NP_000066.1
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Features and Benefits
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Physical form
Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Xiaohan Qu et al.
Journal of translational medicine, 13, 301-301 (2015-09-17)
PIWI proteins have important roles in tumorigenesis due to their interaction with piRNAs. Recent studies suggest that PIWI proteins affect prognosis of various cancers. In the present study, PIWI genes expression was assayed in non-small cell lung cancer (NSCLC). To
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