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Merck
CN

SAB4301135

Anti-FLAG Tag antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
67
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

1.4 mg/mL

technique(s)

western blot: 1:1000-1:5000 (Cell Lysate)

isotype

IgG

immunogen sequence

DYKDDDDK conjugated to KLH (synthetic peptide)

shipped in

wet ice

storage temp.

−20°C

General description

The FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins.

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Application

Anti-FLAG Tag antibody produced in rabbit has been used in:
  • western blotting
  • co-immunoprecipitation
  • immunofluorescence studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

The antibody detects transfected proteins contanining FLAG tag.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料
常规特殊物品

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Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling
Cao Y, et al.
Biochimica et Biophysica Acta, 1863(12), 3148-3159 (2016)
Haijie Li et al.
Journal of experimental & clinical cancer research : CR, 39(1), 12-12 (2020-01-15)
Histone lysine demethylase 4B (KDM4B) has been implicated in various pathological processes and human diseases. Glucose metabolism is the main pattern of energy supply in cells and its dysfunction is closely related to tumorigenesis. Recent study shows that KDM4B protects
Xuan Yang et al.
Cancer science, 110(7), 2133-2144 (2019-05-09)
Lymphoid-specific helicase (LSH) is overexpressed in tumor tissues and its overexpression is associated with poor prognosis in several cancers. However, the role and molecular mechanism of LSH in hepatocellular carcinoma (HCC) remains largely unknown. Herein, we report that LSH was



Global Trade Item Number

SKUGTIN
SAB4301135-100UL04061837435935