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SAB4301254

Sigma-Aldrich

Anti-phospho-PKC α (pTyr657) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
KPCA, PKC III, PKC-A, PKC-alpha
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

80 kDa

species reactivity

human

concentration

1.0 mg/mL

technique(s)

western blot: 1:500-1:1000 (Cell Lysate)

isotype

IgG

accession no.

NP_002728.1.

UniProt accession no.

P17252

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr657)

Gene Information

human ... PRKCA(5578)

General description

Protein kinase C α (PRKCA) is encoded by the gene mapped to human chromosome 17q24.2. The encoded protein is a member of the serine/threonine-specific protein kinase family.

Specificity

The antibody detects endogenous levels of PKC α only when phosphorylated at tyrosine 658.

Immunogen

Peptide sequence around phosphorylation site of tyrosine 658(F-S-Y(p)-V-N) derived from Human PKC α.

Biochem/physiol Actions

Protein kinase C α (PRKCA) is a serine-threonine kinase. It is associated with tumorigenesis, invasion, apoptosis, differentiation, angiogenesis and metastasis. Downregulation of PKCA inhibits the invasion of urinary bladder, colon, renal cell, breast carcinomas, multiple myeloma, glioma and endometrial cancer cells. In endothelial cells, PRKCA-mediated phosphorylation of TRPC1 (transient receptor potential cation channel subfamily C member 1) regulates store operated Ca2+ entry.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Inhibition of protein kinase Calpha prevents endothelial cell migration and vascular tube formation in vitro and myocardial neovascularization in vivo.
Wang A, et al.
Circulation Research, 90, 609-616 (2002)
MZF-1/Elk-1 Complex Binds to Protein Kinase C? Promoter and Is Involved in Hepatocellular Carcinoma.
Yue CH, et al.
PLoS ONE, 10 (2015)
PKC? promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness.
Vaskovicova K, et al.
BMC Cancer (2015)
Protein kinase Calpha phosphorylates the TRPC1 channel and regulates store-operated Ca2+ entry in endothelial cells.
Ahmmed GU, et al.
The Journal of Biological Chemistry, 279, 20941-20949 (2004)
Reduction of protein kinase C α (PKC-α) promote apoptosis via down-regulation of Dicer in bladder cancer.
Jiang Z, et al.
Journal of Cellular and Molecular Medicine, 19, 1085-1093 (2015)
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