SAB4503781
Anti-phospho-Smad3 (pSer425) antibody produced in rabbit
affinity isolated antibody
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, IHC, WB
Citations:
8
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 48 kDa
species reactivity
human, rat, mouse
concentration
~1 mg/mL
technique(s)
ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer425)
Gene Information
human ... SMAD3(4088)
General description
SMAD3 (SMAD family member 3) is located on human chromosome 15q22. Smad 3 belongs to the mothers against Dpp (MAD) related family of proteins.
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425.
Immunogen Range: 376-425
Immunogen Range: 376-425
Application
Anti-phospho-Smad3 (pSer425) antibody has been used in immunoprecipitation and immunoblotting.
Biochem/physiol Actions
SMAD (homologues of the Drosophila protein, mothers against decapentaplegic (Mad) and the Caenorhabditis elegans protein Sma) proteins play an important role in the intracellular signalling of transforming growth factor β (TGFβ). Reduction in the phosphorylation level of Smad3 helps autophagy to control the endothelial-mesenchymal transition. In human arterial smooth muscle cells, suppressing siRNA of SMAD3 improves the viability of cells.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
常规特殊物品
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Find documentation for the products that you have recently purchased in the Document Library.
XBP 1-Deficiency Abrogates Neointimal Lesion of Injured Vessels Via Cross Talk With the PDGF Signaling
Zeng L, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 35(10), 2134-2144 (2015)
TbetaRI phosphorylation of Smad2 on Ser465 and Ser467 is required for Smad2-Smad4 complex formation and signaling
Abdollah S, et al.
The Journal of Biological Chemistry, 272(44), 27678-27685 (1997)
Functional Analysis of a Novel Genome-Wide Association Study Signal in SMAD3 That Confers Protection From Coronary Artery Disease
Turner AW, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 36(5), 972-983 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SAB4503781-100UG | 04061832521442 |