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Merck
CN

SAB4702106

Anti-Dendra2 produced in rabbit

Synonym(s):

Dendra2 tag

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About This Item

UNSPSC Code:
12352203
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conjugate

unconjugated

Quality Level

antibody product type

primary antibodies

clone

PAb (836), recombinant monoclonal

form

buffered aqueous solution

species reactivity

(Species independent)

concentration

1 mg/ml

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Dendra2 is an improved version of green-to-red photoswitchable protein Dendra, and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At hight intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.

Immunogen

Dendra2 tag

Biochem/physiol Actions

The rabbit polyclonal antibody PAb (836) recognizes Dendra2 tag, a green-to red photoswitchable protein derived from an octocoral (Dendronephthya).

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified by protein-A affinity chromatography.
Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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