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Merck
CN

SAB5900004

Alpaca Anti-HA single domain antibody, magnetic beads

recombinant Alpaca(VHH) Nano antibody magnetic bead suspension

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About This Item

NACRES:
NA.41
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biological source

alpaca

Quality Level

recombinant

expressed in Pichia pastoris

conjugate

magnetic beads conjugate

antibody product type

primary antibodies

clone

recombinant monoclonal

form

liquid

technique(s)

ChIP: suitable, RIP: suitable, co-immunoprecipitation (co-IP): suitable, immunoprecipitation (IP): suitable, mass spectrometry (MS): suitable

color

black

matrix

(~45 um (cross-linked to 7.5% magarose beads))

isotype

VHH

capacity

binding capacity (10 μL slurry bind about 2ug-3μg of HA tagged protein)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

HA-tag (YPYDVPDYA) has been extensively used as a general epitope tag, which does not generally interfere with the bioactivity or the biodistribution of the recombinant protein. This tag greatly facilitates the detection, isolation and purification of the protein of interest.Anti-HA single domain antibody, magnetic beads are magnetic agarose spheres covalently coupled with VHH antibodies acquiring high specificity and affinity for HA tag. These beads can efficiently capture and separate HA tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.

Immunogen

HA Tagged Protein

Biochem/physiol Actions

Selectively recognizes HA fusion protein

Features and Benefits

  • Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
  • They are directly coupled to beads covalently.
  • They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
  • No heavy and light chains in downstream interactions.
  • Acids and alkali resistance, tolerance to harsh conditions.
  • Short incubation time 5 – 30 minutes.
  • Low batch to batch variations.
  • Typically, antibodies are not detectable in Western Blot.

Packaging

plastic tube

Physical form

1XPBS, 0.03% sodium azide, 50% glycerol.

Other Notes

Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

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Global Trade Item Number

SKUGTIN
SAB5900004-500UL04065272399692