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SEP030

Seppro™ IgY14

LC5

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Clone:
-
Species reactivity:
-
Application:
—
Citations:
3
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column D × L

12.7 mm × 39.5 mm

capacity

5 mg total protein loading(71 μL of human plasma, average human protein concentration 70 mg/mL)

storage temp.

2-8°C

General description

Seppro™ IgY14 columns allow users to separate 14 highly abundant proteins (HAP) from plasma protein mixtures. The Avian polyclonal IgY (Immunoglobulin Yolk) antibodies, have a distinct, highly specific partitioning advantage over competing products, allowing the researcher to look at a much cleaner human protein mixture. Seppro™ columns make further proteomics analysis into the plasma proteome much easier and provide cleaner results.

Proteins Depleted:

Albumin
IgG
α1-Antitrypsin
IgA
IgM
Transferrin
Haptoglobin
α2-Macroglobulin
Fibrinogen
Complement C3
α1-Acid Glycoprotein (Orosomucoid)
HDL (Apolipoproteins A-I and A-II)
LDL (mainly Apolipoprotein B)

Application

Seppro™ IgY14, LC5 (5 mL bed volume columns) is designed for use as a plasma/serum protein removal tool base upon immune affinity removal of 14 highly abundant proteins (HAP) from human plasma protein mixtures via liquid chromatography. Seppro™ IgY14 is used to support the analysis of low abundance human serum/plasma proteins.

Legal Information

Seppro is a trademark of Merck KGaA, Darmstadt, Germany

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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • S4199
    Seppro™ Dilution buffer
    SDS
  • S4324
    Seppro™ Stripping Buffer
    SDS
  • S4449
    Seppro™ Neutralization Buffer
    SDS
  • CLS8163
    Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile
    SDS

Storage Class

10 - Combustible liquids

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBZ4109
SLCB6761
SLBX1276
SLBS8342
SLBQ8222V

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Related Content

R L Conhaim et al.
American journal of respiratory and critical care medicine, 153(2), 677-683 (1996-02-01)
Lung liquid conductance (Kf) is calculated as the quotient of lung lymph flow divided by net filtration pressure (Pnf), where Pnf is the balance of osmotic and hydrostatic pressures in the lung microcirculation. In protein depletion, lymph flow rises with
Does plasma protein depletion increase lung liquid conductance?
P Dodek
American journal of respiratory and critical care medicine, 154(4 Pt 1), 1213-1215 (1996-10-01)
Renato Millioni et al.
PloS one, 6(5), e19603-e19603 (2011-05-17)
To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number

Global Trade Item Number

SKUGTIN
SEP030-1KT04061837018008