SML1734
FHZ
≥98% (HPLC)
Synonym(s):
2-Amino-3′-hydroxy-6′-(2-(2-(2-hydroxyethoxy)ethoxy)ethoxy)spiro[isoindoline-1,9′-xanthen]-3-one
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About This Item
Empirical Formula (Hill Notation):
C26H26N2O7
CAS Number:
Molecular Weight:
478.49
UNSPSC Code:
12352200
Product Name
FHZ, ≥98% (HPLC)
SMILES string
OC1=CC(OC(C=C(OCCOCCOCCO)C=C2)=C2C34N(N)C(C5=C4C=CC=C5)=O)=C3C=C1
assay
≥98% (HPLC)
form
powder
storage condition
protect from light
color
white to light brown
solubility
DMSO: 30 mg/mL, clear
storage temp.
2-8°C
Biochem/physiol Actions
FHZ is a highly selective, cell-permeable, non-cytotoxic, non-fluorescent 6-triethylene glycol-substituted fluorescein hydrazide that is an efficient fluorescent turn-on probe for dual channel detection of (•OH) and HOCl in live cells and zebrafish embryos.
FHZ is a highly selective, cell-permeable, non-cytotoxic, non-fluorescent 6-triethylene glycol-substituted fluorescein hydrazide that is an efficient fluorescent turn-on probe for dual channel detection of (•OH) and HOCl in live cells and zebrafish embryos. FHZ selectively reacts with hydroxyl (•OH) and HOCl, respectively, to form highly fluorescent FOBA (Ex: 410 nm & Em.: 486 nm; Φ = 0.42; ε410 = 12000/M/cm) and F-TEG (Ex: 490 nm & Em.: 520 nm; Φ = 0.34; ε490 = 7000/M/cm), while exhibiting no reactivity toward H2O2 1O2, O2•–, NO, or ONOO–. Cyan and green fluorescence dual-channel detection with two exciting wavelengths (405 nm and 488 nm) and two collection windows (430–490 nm; 510–560 nm) allows time-dependent quantitative detection of cellular •OH and HOCl by FHZ in live cells (50-100 μM; HeLa and RAW 264.7 macrophages) cells and zebrafish embryos (50 μM).
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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