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Merck
CN

SML4034

BODIPY 630/650 X NHS ester

new

≥85% (HPLC)

Synonym(s):

(T-4)-[2,5-Dioxo-1-pyrrolidinyl 6-[[2-[4-[2-[5-[[5-(2-thienyl)-2H-pyrrol-2-ylidene-κN]methyl]-1H-pyrrol-2-yl-κN]ethenyl]phenoxy]acetyl]amino]hexanoato]difluoroboron, BODIPY 630/650X, BODIPY 630/650 NHS Ester, Bodipy 630/650 succinimidyl ester, BODIPY-X 630/650-NHS ester, BODIPY 630/650X SE

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About This Item

Empirical Formula (Hill Notation):
C33H31BF2N4O6S
CAS Number:
Molecular Weight:
660.50
MDL number:
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Quality Level

Assay

≥85% (HPLC)

form

powder

color

purple to black

solubility

DMSO: 2 mg/mL, clear

storage temp.

-10 to -25°C

SMILES string

O=C(NCCCCCC(ON1C(CCC1=O)=O)=O)COC(C=C2)=CC=C2/C=C/C3=CC=C4N3[B-](F)(F)[N+]5=C(C6=CC=CS6)C=CC5=C4

Biochem/physiol Actions

Amine reactive, red fluorescent labeling reagent that can be used to tag antibodies, proteins, peptides, lipids, and nucleic acids.

BODIPY-X 630/650-NHS ester, a derivative of Boron-dipyrromethene (BODIPY), features a seven-atom aminohexanoyl spacer, is a versatile, amine-sensitive red fluorescent labeling reagent useful to tag antibodies, proteins, peptides, lipids, and nucleic acids. BODIPY 630/650 X is characterized by low toxicity and can be excited at approximately 630 nm, emitting fluorescence at around 650 nm. BODIPY 630/650X stands out due to its high molar extinction coefficient, impressive quantum yield, and an extended lifetime of its excited state. Furthermore, BODIPY-X 630/650 is notable for its brightness, remarkable photostability, and distinct emission spectrum. These properties minimize spectral overlap with other fluorophores and enhance its utility in multiplex imaging, where multiple fluorophores are used simultaneously.

Regulatory Information

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Corinna G Gruber et al.
RSC medicinal chemistry, 11(7), 823-832 (2021-01-23)
A series of fluorescent dibenzodiazepinone-type muscarinic acetylcholine M2 receptor (M2R) ligands was synthesized using various fluorescent dyes (5-TAMRA, λ ex/λ em ≈ 547/576 nm; BODIPY 630/650, λ ex/λ em ≈ 625/640 nm; pyridinium dye Py-1, λ ex/λ em ≈ 611/665
Thomas M D Sheard et al.
Nanoscale, 15(45), 18489-18499 (2023-11-09)
Amine-reactive esters of aromatic fluorescent dyes are emerging as imaging probes for nondescript staining of cellular and tissue architectures. We characterised the staining patterns of 14 fluorescent dye ester species with varying physical and spectral properties in the broadly studied
Joachim Pfister et al.
Scientific reports, 10(1), 15519-15519 (2020-09-25)
Live-cell imaging allows the in vivo analysis of subcellular localisation dynamics of physiological processes with high spatial-temporal resolution. However, only few fluorescent dyes have been custom-designed to facilitate species-specific live-cell imaging approaches in filamentous fungi to date. Therefore, we developed

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