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Merck
CN

SML4162

ACMA, Fluorescent Dye

new

≥95% (HPLC)

Synonym(s):

9-Amino-6-chloro-2-methoxyacridine, 9-Amino-3-chloro-7-methoxyacridine, 6-Chloro-2-methoxy-9-acridinamine, 2-Methoxy-6-chloro-9-aminoacridine, 3-Chloro-7-methoxy-9-aminoacridine, 6-Chloro-9-amino-2-methoxyacridine, DNA Intercalator ACMA, 9-ACMA, NSC 15300

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Quality Level

100

Assay

≥95% (HPLC)

form

powder

color

, Faint green to light yellow brown

solubility

DMSO: soluble mg/mL, clear

storage temp.

-10 to -25°C

SMILES string

ClC(C=CC1=C2N)=CC1=NC3=C2C=C(OC)C=C3

Biochem/physiol Actions

pH-sensitive fluorophore, DNA intercalator, membrane-interactive dye.
ACMA is a versatile fluorescent molecule that exhibits pH-sensitive fluorescence, with high intensity at physiological pH and quenching upon protonation, coupled with decreased membrane permeability. 9-ACMA functions as a DNA intercalator, preferentially binding to poly(dA-dT) sequences over poly(dG-dC), with excitation/emission maxima around 410/480 nm, compatible with various UV light sources. ACMA interacts with energized membranes and undergoes fluorescence quenching in response to transmembrane pH gradients. It has been extensively employed to monitor cation and anion movement across membranes and to study the proton-pumping activity of various membrane-bound ATPases.

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000460882
0000460882
0000449296
0000449296

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Hiofan Hoi et al.
Journal of biotechnology, 281, 99-105 (2018-07-08)
Channelrhodopsins (ChRs) are a group of membrane proteins that allow cation flux across the cellular membrane when stimulated by light. They have been emerged as important tools in optogenetics where light is used to trigger a change in the membrane
Takatoshi Sekiguchi et al.
The Journal of biological chemistry, 300(9), 107659-107659 (2024-08-12)
Chloroplast ATP synthase (CFoCF1) synthesizes ATP by using a proton electrochemical gradient across the thylakoid membrane, termed ΔμH+, as an energy source. This gradient is necessary not only for ATP synthesis but also for reductive activation of CFoCF1 by thioredoxin
Alex Green Wielandt et al.
Methods in molecular biology (Clifton, N.J.), 1377, 171-180 (2015-12-24)
The activity of enzymes involved in active transport of matter across lipid bilayers can conveniently be assayed by measuring their consumption of energy, such as ATP hydrolysis, while it is more challenging to directly measure their transport activities as the

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