Quality Segment
assay
≥98% (HPLC)
form
powder
color
faint green to dark yellow
solubility
DMSO: 2 mg/mL, clear (warmed)
storage temp.
-10 to -25°C
SMILES string
O=C(C1=CN=C2C=CC(C)=CC2=C1NC3=CC=C(N(CC)CC)C=C3)OCC
Application
MCU-i4 may be used as a mitochondrial calcium uniporter (MCU) inhibitor to study its effects on cell viability, apoptosis, cytosolic Ca²⁺ levels, mitochondrial Ca²⁺ and membrane potential, glycolytic rate, ATP production, and reactive oxygen species generation in breast cancer cells.
Biochem/physiol Actions
MCU-i4 is a mitochondrial calcium uniporter (MCU) inhibitor that selectively suppresses MCU complex-mediated mitochondrial Ca2+ uptake via direct interaction with MICU1. MCU-i4 (10 μM) effectively inhibits agonist-induced mitochondria Ca2+ uptake in intact cells (agonist = 100 μM histamine when using HeLa, 100 μM ATP when using MEF, MDA-MB-231, and HEK293A), exhibiting no effect in cells expressing MICU1 mutant construct (Q302A, Q306A, L443A) that does not bind MCU-i4 or in MICU1-, but not MICU2-, silenced cells.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable