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SML4178

Maleimide-PEG2-biotin

Name: Maleimide-PEG2-biotin
Brand: SIGMA

≥98% (HPLC), powder, biotinylation reagent

Synonym(s):

(3aS,4S,6aR)-N-[2-[2-[2-[[3-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-1-oxopropyl]amino]ethoxy]ethoxy]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, Biotin-PEG2-Mal, Biotin-PEO-maleimide

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About This Item

Empirical Formula (Hill Notation):

C₂₃H₃₅N₅O₇S

CAS Number:

305372-39-8

Molecular Weight:

525.62

MDL number:

MFCD09264743

NACRES:

NA.21

Assay:

≥98% (HPLC)

Form:

powder

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Properties

Quality Segment

100

assay

≥98% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 2 mg/mL, clear

storage temp.

-10 to -25°C

SMILES string

O=C(N1CCC(NCCOCCOCCNC(CCCC[C@H]2[C@@]3([C@]([H])(CS2)NC(N3)=O)[H])=O)=O)C=CC1=O

Description

General description

Maleimide-PEG2-biotin, a maleimide-conjugated biotin molecule with a medium-length spacer arm (PEG)2, is membrane-impermeable and exhibits high affinity for free thiol groups on cysteines.

Application

Maleimide-PEG2-biotin is suitable for use in labeling and purifying biomolecules with high specificity. It may be used in mass spectrometry to detect free sulfhydryl residues in intact proteins.

Biochem/physiol Actions

Biotin-PEG2-Mal, a biotin maleimide linker, forms stable thioether bonds by covalently labeling cysteine sulfhydryl groups on antibodies or proteins and can be detected via streptavidin-HRP in Western blot assays following pull-down. Maleimide labels react with sulfhydryl (–SH) groups at acidic pH, minimizing the risk of disulfide bond scrambling during labeling that can happen at higher pH levels.

pictograms

GHS07

signalword

Warning

hcodes

H302

pcodes

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c