Skip to Content
Merck
CN

SRE0018

Lyticase from Arthrobacter Luteus

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

Synonym(s):

Lyticase from Arthrobacter luteus

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
37340-57-1
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
MFCD00131560

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Lyticase from Arthrobacter Luteus, Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

color

light brown

biological source

bacterial (Arthrobacter luteus)

form

lyophilized powder

specific activity

≥2000 units/mg protein

composition

Protein, ≥20% biuret

concentration

20 % protein Biuret

Quality Level

400

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Other Notes

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Application

For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).

General description

Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000480212
0000480211
0000480210
0000480212
0000480210

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Louis Dacquay et al.
G3 (Bethesda, Md.), 7(6), 1799-1809 (2017-04-30)
Actively proliferating cells constantly monitor and readjust their metabolic pathways to ensure the replenishment of phospholipids necessary for membrane biogenesis and intracellular trafficking. In Saccharomyces cerevisiae, multiple studies have suggested that the lysine acetyltransferase complex NuA4 plays a role in

Protocols

Enzymatic Assay of Lyticase

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

溶壁酶的酶学测定

本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service