SRE0018
Lyticase from Arthrobacter Luteus
Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein
Synonym(s):
Lyticase from Arthrobacter luteus
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About This Item
Specific activity:
≥2000 units/mg protein
Biological source:
bacterial (Arthrobacter luteus)
Concentration:
20 % protein Biuret
color
light brown
biological source
bacterial (Arthrobacter luteus)
form
lyophilized powder
specific activity
≥2000 units/mg protein
composition
Protein, ≥20% biuret
concentration
20 % protein Biuret
Quality Level
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
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General description
Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.
Application
For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Features and Benefits
Features:
- Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
- Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
- M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
- Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
- Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
- Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
- Site quality assessment
- ISO 9001 certificate
- Detailed product specifications
- Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
- Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
- Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
- Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
- Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
- Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
- Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Other Notes
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
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Louis Dacquay et al.
G3 (Bethesda, Md.), 7(6), 1799-1809 (2017-04-30)
Actively proliferating cells constantly monitor and readjust their metabolic pathways to ensure the replenishment of phospholipids necessary for membrane biogenesis and intracellular trafficking. In Saccharomyces cerevisiae, multiple studies have suggested that the lysine acetyltransferase complex NuA4 plays a role in
Protocols
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。
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