Lyticase from Arthrobacter Luteus

Lyticase is an enzyme obtained from Arthrobacter Luteus that can extract DNA from yeast by inducing partial spheroplast formation which are later lysed to release DNA. It is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Lyticase contains β-(1→3)-glucan laminaripentaohydrolase with additional β-(1→3)-glucanase, protease, and mannanase activities.

For isolation of nucleic acids, lyticase is used in the lysis of yeast cell walls (e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species).

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer