SRP0167
DNMT3B Active from mouse
recombinant, expressed in baculovirus infected insect cells, ≥30% (SDS-PAGE)
Synonym(s):
DNA (cytosine-5-)-methyltransferase 3b, DNA MTase MmuIIIB
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
mouse
recombinant
expressed in baculovirus infected insect cells
Assay
≥30% (SDS-PAGE)
form
aqueous solution
mol wt
99 kDa
packaging
pkg of 10 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
mouse ... DNMT3B(13436)
General description
Mouse DNMT3B (DNA methyltransferase 3 beta) belongs to the murine Dnmt3 family. It is expressed at high levels in undifferentiated ES cells. Gene is located on chromosome 20 ICF locus. DNMT3B codes for a functional DNA methyltransferase. In human, DNMT3B gene is mapped to chromosome 20q11.2. In mouse, Dnmt3b gene is located on the chromosome 20 ICF locus.
Application
Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
DNMT3B (DNA methyltransferase 3 beta) controls DNA methylation. Mutations in DNMT3B results in ICF syndrome (immunodeficiency, centromeric instability, facial anomalies). Dnmt3b is required for de novo methylation and mammalian development. It is also essential for methylation of centromeric minor satellite repeats.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Regulatory Information
常规特殊物品
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The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome.
Hansen RS, et al.
Proceedings of the National Academy of Sciences of the USA, 96(25), 14412-14417 (1999)
Increased DNA methyltransferase 3b (Dnmt3b)-mediated CpG island methylation stimulated by oxidative stress inhibits expression of a gene required for neural tube and neural crest development in diabetic pregnancy.
Wei D & Loeken MR
Diabetes, 63(10), 3512-3522 (2014)
DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.
Okano M, et al.
Cell, 99(3), 247-257 (1999)
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