SRP0443
UCHL3 active human
recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)
Synonym(s):
UCHL3, Ubiquitin Carboxy-terminal Hydrolase L3
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf9 cells
Assay:
≥90% (SDS-PAGE)
Form:
aqueous solution
Mol wt:
27.1 kDa
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
27.1 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
2.07 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... UCHL3(7347)
General description
UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002) amino acids 2-231 (end) with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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H Wada et al.
Biochemical and biophysical research communications, 251(3), 688-692 (1998-10-29)
NEDD8 is a novel ubiquitin-like protein that has been shown to conjugate to nuclear proteins in a manner analogous to ubiquitination and sentrinization. To identify proteins that are involved in the NEDD8-conjugation and de-conjugation pathway, the yeast two-hybrid system was
Nam, M.J., et al.
Proteomics, 3, 2108-2115 (2004)
Rieko Setsuie et al.
Neurochemistry international, 54(5-6), 314-321 (2009-01-22)
Ubiquitin (Ub) carboxy terminal hydrolase (UCH)-L1 and UCH-L3 are two of the deubiquitinating enzymes expressed in the brain. Both gad mice, which lack UCH-L1 expression and Uchl3 knockout mice exhibit neurodegeneration, although at distinct areas. These phenotypes indicate the importance
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