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SRP0443

Sigma-Aldrich

UCHL3 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Synonym(s):

UCHL3, Ubiquitin Carboxy-terminal Hydrolase L3

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Key Documents

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biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

27.1 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

2.07 mg/mL

NCBI accession no.

NM_006002

UniProt accession no.

P15374

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UCHL3(7347)

General description

UCHL3, also known as Ubiquitin Carboxy-terminal Hydrolase L3 (GenBank Accession No. NM_006002) amino acids 2-231 (end) with N-terminal His-tag, MW=27.1 kDa, expressed in E. coli.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
8035300826

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Nam, M.J., et al.
Proteomics, 3, 2108-2115 (2004)
H Wada et al.
Biochemical and biophysical research communications, 251(3), 688-692 (1998-10-29)
NEDD8 is a novel ubiquitin-like protein that has been shown to conjugate to nuclear proteins in a manner analogous to ubiquitination and sentrinization. To identify proteins that are involved in the NEDD8-conjugation and de-conjugation pathway, the yeast two-hybrid system was
Rieko Setsuie et al.
Neurochemistry international, 54(5-6), 314-321 (2009-01-22)
Ubiquitin (Ub) carboxy terminal hydrolase (UCH)-L1 and UCH-L3 are two of the deubiquitinating enzymes expressed in the brain. Both gad mice, which lack UCH-L1 expression and Uchl3 knockout mice exhibit neurodegeneration, although at distinct areas. These phenotypes indicate the importance

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