SRP3015
Beta NGF human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture
Synonym(s):
beta-Nerve Growth Factor
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (SDS-PAGE)
form
lyophilized
potency
<1.0 ng/mL ED50
mol wt
(Noncovalent Homodimer, 13.6 kDa (121 amino acids))
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NGF(4803)
General description
β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human β-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.
Application
β NGF human has been used in immunohistochemistry.
Biochem/physiol Actions
β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The functional form of human b-NGF is a noncovalently disulfide-linked homodimer, of two 13.5 kDa polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.
Physical form
Lyophilized from a 0.2 μm filtered solution containing0.1% Trifluoroacetic Acid (TFA)
Preparation Note
Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
Other Notes
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Huiyu Chen et al.
Journal of inflammation (London, England), 16, 27-27 (2020-01-01)
In herpes simplex epithelial keratitis, excessive TLR3-induced cellular responses after virus infection evoke inflammatory cascades that might be destructive to the host cornea. Nerve growth factor (NGF), a pluripotent neurotrophic factor with immune regulatory effect, was proved to be effective
The nerve growth factor 35 years later.
R Levi-Montalcini
Science (New York, N.Y.), 237(4819), 1154-1162 (1987-09-04)
H Zhang et al.
European journal of histochemistry : EJH, 59(3), 2522-2522 (2015-10-03)
The nerve growth factor (NGF) not only has an essential effect on the nervous system, but also plays an important role in a variety of non-neuronal systems, such as the reproductive system. The aim of this study was to investigate
Rui Li et al.
Theranostics, 10(4), 1649-1677 (2020-02-12)
Rationale: Autophagy in Schwann cells (SCs) is crucial for myelin debris degradation and clearance following peripheral nerve injury (PNI). Nerve growth factor (NGF) plays an important role in reconstructing peripheral nerve fibers and promoting axonal regeneration. However, it remains unclear
A Ullrich et al.
Nature, 303(5920), 821-825 (1983-06-30)
Nerve growth factor (NGF) is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones (see refs 1-3 for review). NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three
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