SRP3050
Granulocyte-Macrophage Colony-Stimulating Factor human
GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
Synonym(s):
CSF-2, MGI-1GM, pluripoietin-a
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.1 ng/mL
mol wt
14.6 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CSF2(1437)
General description
GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. Recombinant human GM-CSF is a 14.6kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
Application
Granulocyte-Macrophage Colony-Stimulating Factor human has been used in the medium for plating of the cells. It has been used in culture medium for the differentiation of human peripheral blood mononuclear cells.
Biochem/physiol Actions
GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration and enhances the functional activity of the mature end-cells.
Physical form
Lyophilized from 10 mM Sodium Citrate, pH 3.5.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Other Notes
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Siddappa N Byrareddy et al.
PloS one, 10(10), e0140689-e0140689 (2015-10-16)
Lectin-like molecules and their receptors are cell surface molecules that have been shown to play a role in either facilitating infection or serving as transporters of HIV/SIV in vivo. The role of these lectin-like molecules in the pathogenesis of HIV/SIV
Candida parapsilosis produces prostaglandins from exogenous arachidonic acid and OLE2 is not required for their synthesis.
Grozer Z, et al.
Virulence, 6, 85-92 (2015)
Rapamycin inhibits GM-CSF-induced neutrophil migration.
Gomez-Cambronero J
Febs Letters, 550, 94-100 (2003)
Growth of clonogenic myeloblastic leukemic cells in the presence of human recombinant erythropoietin in addition to various human recombinant hematopoietic growth factors.
Asano Y
Blood, 72, 1682-1686 (1988)
Association of genetic variations in the CSF2 and CSF3 genes with lung function in smoking-induced COPD.
He JQ
The European Respiratory Journal, 32, 25-34 (2008)
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