SRP3087
IL-21 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
IL-21 Protein
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.5 ng/mL ED50
mol wt
15.4 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL21(59067)
General description
IL21 (interleukin 21) is a member of the γ-chain type I cytokine family, and a four-α-helix bundle pleiotropic cytokine. This gene is localized to human chromosome 4q26–q27. Although, it is predominantly produced by T follicular helper (TFH) and T helper-17 (TH17) cells, it is also produced by CD4+ T cells, neutrophils and natural killer cells. Under certain conditions, it is also produced by CD8+ T cells. Recombinant human IL-21 is a 15.0 kDa protein consisting of 132 amino acid residues.
Biochem/physiol Actions
IL21 (interleukin 21) functions on its heterodimeric receptor IL21R, which is expressed widely on immune and non-immune cells. Therefore, IL21 exerts its effects on a wide range of cell types and has a pleiotropic role in immune response regulation. This protein activates Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway and functions through mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)-AKT signaling pathways. Studies show that in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS), IL21 facilitates migration, invasion and production of MMPs (MMP-2, MMP-3, MMP-9, MMP-13) (matrix metalloproteinases). Therefore, it has potential as a therapeutic target in the treatment of RA. Studies show that CD3+TCRvβ11+ NK (natural killer) T cells isolated from a local Mycobacterium tuberculosis infection site produce IL21, which in turn assists B cells to produce and release IgG (immunoglobulin) and IgA, to help fight the infection.
Physical form
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Other Notes
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Interleukin-21 induces migration and invasion of fibroblast-like synoviocytes from patients with rheumatoid arthritis.
Xing R, et al.
Clinical and Experimental Immunology, 184(2), 147-158 (2016)
Biological effects of IL-21 on different immune cells and its role in autoimmune diseases.
Gharibi T, et al.
Immunobiology, 221(2), 357-367 (2016)
Antigen-specific human NKT cells from tuberculosis patients produce IL-21 to help B cells for the production of immunoglobulins.
Wu C, et al.
Oncotarget, 6(30), 28633-28645 (2015)
Julia Parrish-Novak et al.
Journal of leukocyte biology, 72(5), 856-863 (2002-11-14)
Interleukin (IL)-21 was recently discovered using a functional cloning approach based on expression of its receptor. It is similar in domain organization and primary sequence to IL-2 and IL-15. Like these cytokines, IL-21 uses the common gamma chain of the
Hamza Loucif et al.
Autophagy, 18(6), 1256-1273 (2021-10-07)
The maintenance of a strong IL21 production in memory CD4 T cells, especially in HIV-1-specific cells, represents a major correlate of natural immune protection against the virus. However, the molecular mechanisms underlying IL21 production during HIV-1 infection, which is only
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