SRP3270
TWEAK human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
Apo3-Ligand, DR3LG, TNF-related weak inducer of apoptosis receptor, TNFSF12
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
30-45 ng/mL ED50
mol wt
17.0 kDa
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TNFSF12(8742)
General description
TWEAK (TNF-related weak inducer of apoptosis) belongs to the TNF (tumor necrosis factor) family of ligands. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. It exists in two active forms: membrane-associated mTWEAK and soluble type sTWEAK. The TWEAK gene is mapped to human chromosome 17p13.1. The protein has an amino terminal hydrophobic region.
Recombinant human TWEAK is a soluble 17.0kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.
Recombinant human TWEAK is a soluble 17.0kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.
Biochem/physiol Actions
TWEAK (TNF-related weak inducer of apoptosis) binds to fibroblast growth factor-inducible 14 (Fn14). It is mainly involved in nuclear factor κB (NF-κB) pathway and is responsible for cellular proliferation, migration, cell death, differentiation, angiogenesis and inflammation. TWEAK is associated with various disorders, including atherosclerosis, heart failure, type 2 diabetes, ischemic stroke, multiple sclerosis and chronic kidney disease. This gene is upregulated after tissue injury and in tumors.
Physical form
Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 80 mM L-Arginine.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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The role of TWEAK/Fn14 signaling in the MPTP-model of Parkinson's disease.
Mustafa S, et al.
Neuroscience, 319, 116-116 (2016)
Soluble TWEAK and Major Adverse Cardiovascular Events in Patients with CKD.
Fernandez-Laso V, et al.
Clinical journal of the American Society of Nephrology : CJASN, 11, 413-413 (2016)
TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor.
Wiley SR and Winkles JA
Cytokine & Growth Factor Reviews, 14, 241-241 (2003)
Altay M
Altay M, et al.
Endokrynologia Polska, 67, 562-562 (2016)
S R Wiley et al.
Immunity, 15(5), 837-846 (2001-12-01)
TWEAK is a member of the TNF ligand family that induces angiogenesis in vivo. We report cloning of a receptor for TWEAK (TweakR) from a human umbilical vein endothelial cell (HUVEC) library. The mature form of TweakR has only one
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