SRP3292
R-SPONDIN-1 human
recombinant, expressed in CHO cells, ≥95% (SDS-PAGE)
Synonym(s):
Rspo1
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
biological source
human
recombinant
expressed in CHO cells
assay
≥95% (SDS-PAGE)
form
lyophilized
potency
≤50 ng/mL ED50
mol wt
monomer (26.8 kDa (243 amino acids))
packaging
pkg of 20 μg
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... RSPO1(284654)
General description
R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the (Rspo) family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in adrenal glands, ovary, testis, thyroid, and trachea. Recombinant human R-Spondin-1 is a 26.7kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0kDa by SDS PAGE analysis under reducing conditions.
Biochem/physiol Actions
Rspo (R-Spondin) can interact with the Frizzled/LRP6 (LDL receptor related protein 6) receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. Disruption of Rspo-1 gene causes recessive syndrome with XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Rspo-1 is also associated with radioresistance of tumor cells.
Physical form
Lyophilized from a 0.2 μm filtered solution containingPhosphate Buffered Saline (PBS)
Preparation Note
Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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