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SRP4595

Sigma-Aldrich

IFN-alpha 2b human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

IFNA, INFA2, Interferon α 2b

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500 MG
¥10,747.66

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

¥10,747.66


Please contact Customer Service for Availability

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biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

>2.0x10^7 units/mg

mol wt

~19.3 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

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This Item
P3338P3360SRP6058
biological source

human semen

biological source

human semen

biological source

human

biological source

human

assay

≥99% (SDS-GE)

assay

≥95% (SDS-GE)

assay

≥99% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

dry ice

Gene Information

human ... KLK3(354)

Gene Information

human ... KLK3(354)

Gene Information

human ... KLK3(354), SERPINA3(12)

Gene Information

human ... KLK3(354)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−70°C

General description

The gene IFN-α2b (interferon α 2b) is mapped to human chromosome 9. The encoded protein is a type 1 interferon and a glycoprotein. It belongs to the class of cytokines. Human IFN-α2b is a 19.3kDa protein containing 165 amino acid residues.[1][2] Structurally, IFN-α2b is O-glycosylated at the threonine residue at position 106.[3]

Application

IFN-α2b human has been used:
  • as an immunotherapeutic agent to test its antiproliferative and proapoptotic effects in RCC cell lines[4]
  • to stimulate peripheral blood mononuclear cell (PBMC) prior to RNA isolation[5]
  • to stimulate H4 cells before immunoprecipitation[6]
  • to study role of USP18 (ubiquitin specific peptidase 18)-mediation deISGylation in controlling cellular autophagy upon treatment with type I interferons[6]

Biochem/physiol Actions

IFN-α2b (interferon α 2b) is a lymphoid factor that possesses potent anti-viral activity. It mainly interacts with cell surface-specific receptors. This interaction is responsible for anti-pathogenic response in cells. IFN-α2b also exhibits anti-tumor activities. Humans with brain tumor were shown to have mutations in this gene.[1][2] IFN-α2b induces the secretion of cytokine IFN-γ in T cells.[7] The hydrogels embedded with IFN-α2b exploits this functionality in gastric cancer cells.[7] Recombinant IFN-α types are effective against Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in in vitro studies.[8]

Physical form

Lyophilized from 5 mM sodium phosphate, pH 7.4.

Reconstitution

Reconstitute in water to a concentration of 1 mg/mL. This solution can then be diluted into other aqueous buffers. Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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