SRP4623P
Myostatin Proform human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
GDF-8, Growth Differentiation Factor 8, MSTN, MSTN Muscle Hypertrophy
Product Name
Myostatin Proform human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
~27.8 kDa
packaging
pkg of 10 μg
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MSTN(2660)
Biochem/physiol Actions
MSTN (myostatin) functions as a negative modulator of muscle cell development. It functions through forming heteromeric receptor complexes such as with activin type II receptor B (ActRIIB), and with lesser affinity with ActIIA. This interaction results in the recruitment of type I receptors like activin receptor-like kinase 4 (ALK4) or ALK5, leading to the phosphorylation of Smad2 (small mothers against decapentaplegic) and Smad3, which move to the nucleus and suppress myogenesis. In MSTN-null mice, there is delayed skin wound healing due to the increased expression of decorin, an endogenous transforming growth factor-β (TGF β) suppressor. Topical treatment with TGF-β1 restored skin wound healing, suggesting that MSTN agonists might have therapeutic beneficial in skin wound repair.
General description
MSTN (myostatin) belongs to the transforming growth factor-β (TGF β) superfamily containing numerable homologous cytokine proteins. This gene is expressed in skeletal muscle, and codes for a precursor protein composed of an N-terminal leader peptide, a pro-peptide domain, and a C-terminal domain. This precursor protein is proteolytically cleaved by bone morphogenetic protein 1 (BMP-1) at a conserved four amino acid RSRR site to produce the 13kDa mature protein.
Physical form
Lyophilized without any additives.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1 to 1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Myostatin-null mice exhibit delayed skin wound healing through the blockade of transforming growth factor-? signaling by decorin.
Zhang C
American Journal of Physiology. Cell Physiology, 302, 1213-1225 (2012)
Myostatin rapid sequence evolution in ruminants predates domestication.
Tellgren A
Molecular Phylogenetics and Evolution, 33, 782-790 (2004)
Qiqi Yang et al.
Disease models & mechanisms, 12(2) (2019-02-06)
Cancer cachexia affects up to 80% of patients with advanced solid cancer and leads to excessive muscle wasting. Here, using an inducible zebrafish hepatocellular carcinoma (HCC) model driven by oncogenic krasG12V , we observed a progressive muscle-wasting phenotype in adult
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