SRP4895A
C5a (His-Tag) from mouse
recombinant, expressed in E. coli, ≥95% (SDS-PAGE)
Synonym(s):
Complement C5a
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About This Item
biological source
mouse
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
~9 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... C5a(15139)
General description
Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
Biochem/physiol Actions
C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Physical form
Sterile filtered and Lyophilized without additives.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute to a concentration of 1.0 mg/mL in ice-cold water containing 2.5 mg/mL BSA. The solution should be aliquoted and immediately stored at -70°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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John D L, et al.
Immune Responses to Biosurfaces: Mechanisms and Therapeutic Interventions (2015)
Molecular and expression analysis of complement component C5 in the nurse shark (Ginglymostoma cirratum) and its predicted functional role.
Matthew G, et al.
Fish & Shellfish Immunology, 27(1), 40-49 (2009)
Hassan O J Morad et al.
Scientific reports, 12(1), 11078-11078 (2022-07-01)
Immune cell chemotaxis to the sites of pathogen invasion is critical for fighting infection, but in life-threatening conditions such as sepsis and Covid-19, excess activation of the innate immune system is thought to cause a damaging invasion of immune cells
New developments in C5a receptor signaling
Sarma J V and Peter A W
Cell health and cytoskeleton, 4, 73-82 (2012)
Complement System Part II: Role in Immunity
Nicolas S M, et al.
Frontiers in Immunology, 6, 257-257 (2015)
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