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Merck
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SRP5004

AMPK (α2/β2/γ2), active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

AAKG, AAKG2, AMPK, AMPK2, CMH6, H91620p, MGC61468, PRKAA, PRKAA2, PRKAB2, PRKAG2, WPWS

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Key Documents

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recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

255-345 nmol/min·mg

mol wt

~36 kDa (B2)
 ~65 kDa (G2)
 ~69 kDa (A2)

NCBI accession no.

NM_001040633
NM_005399
NM_006252

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKAA2(5563), PRKAB2(5565), PRKAG2(51422)

General description

AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

Physical form

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
N029-2

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Barbara B Kahn et al.
Cell metabolism, 1(1), 15-25 (2005-08-02)
The AMP-activated protein kinase (AMPK) is an evolutionarily conserved sensor of cellular energy status, and recent data demonstrate that it also plays a critical role in systemic energy balance. AMPK integrates nutritional and hormonal signals in peripheral tissues and the
D Grahame Hardie
Journal of cell science, 117(Pt 23), 5479-5487 (2004-10-29)
The AMP-activated protein kinase (AMPK) cascade is a sensor of cellular energy status. Whenever the cellular ATP:ADP ratio falls, owing to a stress that inhibits ATP production or increases ATP consumption, this is amplified by adenylate kinase into a much

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