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SRP6062

Sigma-Aldrich

PP2C α human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

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Synonym(s):
PP1A, PP2CA, PPM1A, Protein phosphatase 1A
CAS Number:
362690-38-8
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥95% (SDS-PAGE)

form

liquid

mol wt

46.6 kDa (418aa)

packaging

pkg of 100 μg

NCBI accession no.

P35813

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PPM1A(5494)

General description

Protein phosphatase 2C-α (PP2C-α), also referred to as protein phosphatase, Mg2+/Mn2+ dependent 1A (PPM1A), is encoded by the gene mapped to human chromosome 4q12. The encoded protein belongs to the family of Ser/Thr protein phosphatases. It is characterized with a catalytic subunit and a constant regulatory subunit. PP2C-α is abundantly expressed in heart and skeletal muscle.

Biochem/physiol Actions

Protein phosphatase 2C-α (PP2C-α) is known to be a negative regulator of cell stress response pathways. Additionally, it has been reported to negatively regulate transforming growth factor-β (TGF-β) signaling pathway and cell cycle. PP2C-α has been shown to dephosphorylate cyclin-dependent kinases (CDKs) and it may be involved in cell cycle control. It modulates cell migration and invasion.

Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW

Physical form

1 mg/mL solution in 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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PPM1A Methylation Is Associated With Vascular Recurrence in Aspirin-Treated Patients.
Gallego-Fabrega C, et al.
Stroke, 47(7), 1926-1929 (2016)
Integrated analysis of expression and genome alteration reveals putative amplified target genes in esophageal cancer.
Takashi S, et al.
Oncology Letters, 18(2), 465-472 (2007)
Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2Cα and β2 isoforms.
Cheng A, et al.
The Journal of Biological Chemistry, 275(44), 34744-34749 (2000)
Loss of PPM1A expression enhances invasion and the epithelial-to-mesenchymal transition in bladder cancer by activating the TGF-β/Smad signaling pathway.
Geng J, et al.
Oncotarget, 5(14), 5700-5711 (2014)
Conformational Changes in Active and Inactive States of Human PP2Cα Characterized by Hydrogen/Deuterium Exchange-Mass Spectrometry.
Mazur S J, et al.
Biochemistry, 56(21), 2676-2689 (2017)
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