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Merck
CN

SRP6176

Jacalin

recombinant, expressed in Artocarpus heterophyllus (jackfruit), ≥97% (SDS-PAGE)

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥97% (SDS-PAGE)
Biological source:
Artocarpus integrifolia seeds
Recombinant:
expressed in Artocarpus heterophyllus (jackfruit)
Mol wt:
14.6 kDa (β chain), 17.1 kDa (full protein)
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biological source

Artocarpus integrifolia seeds

recombinant

expressed in Artocarpus heterophyllus (jackfruit)

assay

≥97% (SDS-PAGE)

form

lyophilized

mol wt

14.6 kDa (β chain), 17.1 kDa (full protein)

packaging

pkg of 5 mg

technique(s)

immunohistochemistry: suitable

impurities

<0.1EU/mg

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

General description

Jacalin is isolated from Jackfruit seeds and purified by affinity chromatography. It is a glycoprotein with two non-covalently bound subunits (14.6 and 17.1 kDa) and belongs to the family of galactose-binding lectins. Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. BioVision′s Jacalin is supplied as a white to light-yellow lyophilized powder without additives. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 14 kDa and 17 kDa. Gene Bank Accession Number Q38720.

Biochem/physiol Actions

Jacalin is one of the two lectins found in jackfruit seeds. The other lectin is artocarpin. Jacalin has been used to inhibit herpes simplex virus type 2. It has been used to assess the immune status of patients infected with human immunodeficiency virus 1 (HIV1) as it exhibits mitogenic effect on human CD4+T lymphocytes. It has been used to analyze 0-linked glycoproteins due to its high specificity for O-glycoside of the disaccharide Thomsen-Friedenreich antigen (Galβ1-3GalNAc).

Physical form

Lyophilized without additives

Preparation Note

Centrifuge the vial prior to opening.
Reconstitute in water.

Other Notes

MAYSSLLSLS VLALLFSISS ADTRKWFLAN GINQNPIGII EAAVGVSEDL LNLNGMEAKN KSQTV IVGPWGAQVS TSSNGKAFDD GAFTGIREIN LSYNKETAIG DFQVIYDLNG SPYVGQNHTS FIKGFTPVKI SLDFPSEYIV DVSGYTGKVS GYVVVRSLTF KTNKKTYGPY GVTSGTPFNL PIENGLVVGF KGSIGYWLDY FSMYLSL

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Jackfruit and its many functional components as related to human health: a review.
Swami SB
Comprehensive Reviews in Food Science and Food Safety, 11, 565-576 (2012)

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