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Merck
CN

SRP6272

SPAM1 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

Synonym(s):

HYA1, HYAL1, HYAL3, HYAL5, PH-20, SPAG15

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200

Key Documents

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Product Name

SPAM1 human, recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

biological source

human

recombinant

expressed in HEK 293 cells

conjugate

(Glycosaminoglycan)

tag

6-His tagged (C-terminus)

assay

≥92% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 52.1 kDa
observed mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.)

packaging

pkg of 10 μg
pkg of 50 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

P38567

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SPAM1(6677)

General description

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1→4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Physical form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
9J297247

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Andrée-Anne Saindon et al.
Reproduction (Cambridge, England) (2018-10-18)
Sperm adhesion molecule 1 (SPAM1) is a sperm protein possessing a hyaluronidase domain in its N-terminus and a zona pellucida-binding domain in its C-terminus. Our previous studies showed that bovine spermatozoa potentially have 2 SPAM1 isoforms that present different C-terminal
Dong Leng et al.
BioMed research international, 2020, 3659451-3659451 (2020-04-08)
Idiopathic pulmonary fibrosis (IPF), the most common interstitial lung disease, arises from transforming growth factor beta 1- (TGFβ1-) induced aberrant fibroproliferation in response to epithelial injury. The TGFβ1-) induced aberrant fibroproliferation in response to epithelial injury. The TGF. We first

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