SRP6293
IL-15 human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
Interleukin-15
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
calculated mol wt 12.8 kDa (no tag), observed mol wt 16 kDa (DTT-reduced. Protein migrates due to glycosylation. Asn 49 is the predicted N-terminus .)
Impurities:
<1 EU/μg endotoxin (LAL test)
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
calculated mol wt 12.8 kDa (no tag), observed mol wt 16 kDa (DTT-reduced. Protein migrates due to glycosylation. Asn 49 is the predicted N-terminus .)
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IL-15(3600)
General description
IL15 (interleukin 15) gene is localized to human chromosome 4q31, composed of eight exons, and the encoded protein is a short-chain α-helical bundle type 1 cytokine. IL15, along with its receptor IL15Rα, are co-expressed majorly by activated monocytes and DCs (dendritic cells).
Biochem/physiol Actions
IL15 (interleukin 15) and IL2 are the only two known cytokines till date that bind to T-cells through IL-2Rβ (IL2 receptor β). It interacts with lymphocytes via the heterotrimeric IL-15 receptor (IL-15R) composed of IL-15Rα, IL-2 receptor β (IL-2Rβ), and IL-2 receptor γ (IL-2Rγ). Since, IL-2R and IL-15R have common subunits, it is supposed that IL2 and IL15 perform similar functions, such as induction of lymphocyte activation and proliferation in vitro, and apoptosis of activated lymphocytes (AICD). However, unlike IL2, IL15 mimics the function of IFN I (interferon) by potently and selectively activating memory-phenotype CD44hi CD8+ cells.
Physical form
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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The shared and contrasting roles of interleukin-2 (IL-2) and IL-15 in the life and death of normal and neoplastic lymphocytes: implications for cancer therapy
Waldmann T A
Cancer Immunology, Immunotherapy, 3(3), 219- 227 (2015)
IL-15 receptor maintains lymphoid homeostasis by supporting lymphocyte homing and proliferation.
Lodolce JP, et al.
Immunity, 9(5), 669-676 (1998)
Potent and selective stimulation of memory-phenotype CD8+ T cells in vivo by IL-15.
Zhang X, et al.
Immunity, 8(5), 591-599 (1998)
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