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SRP6404

Sigma-Aldrich

EGFR/ErbB1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

ERBB, ERBB1, HER1, PIG61, mENA

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 69.5 kDa
observed mol wt 110-115 kDa (DTT-reduced.Protein migrates due to glycosylation.)

packaging

pkg of 10 μg

impurities

<1 EU/μg endotoxin (LAL test)

UniProt accession no.

shipped in

wet ice

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This Item
SRP0242SRP5325SRP0239
assay

≥95% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in HEK 293 cells

recombinant

expressed in baculovirus infected insect cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected insect cells

mol wt

calculated mol wt 69.5 kDa, observed mol wt 110-115 kDa (DTT-reduced.Protein migrates due to glycosylation.)

mol wt

88 kDa

mol wt

~89 kDa

mol wt

87.3 kDa

form

lyophilized

form

aqueous solution

form

buffered aqueous glycerol solution

form

aqueous solution

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

EGFR (epidermal growth factor receptor) belongs to the ErbB family of receptor tyrosine kinases.[1] It is also known as ErbB1/HER1 (human epidermal growth factor receptor). It is located on human chromosome 7p11.2.[2] EGFR has a large extracellular ligand-binding domain, a single transmembrane segment, an intracellular juxtamembrane segment, a tyrosine kinase domain and a carboxy-terminal tail.[1]
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. (provided by RefSeq)

Biochem/physiol Actions

EGFR (epidermal growth factor receptor) plays an important role in ligand binding, dimerization and autophosphorylation.[1] It also plays an important role in several procarcinogenic cancers like gastric cancer. EGFR controls various functions of epithelial cell. It modulates the actions in bacterial infection and is associated with the initiation of macrophage.[3]

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    EGFR regulates macrophage activation and function in bacterial infection
    Hardbower DM, et al.
    The Journal of Clinical Investigation, 126(9), 3296-3312 (2016)
    Synthesis and biochemical characterization of EGF receptor in a water-soluble membrane model system
    Scharadin TM, et al.
    PLoS ONE (2017)
    Chalita Washington et al.
    PLoS genetics, 16(6), e1008715-e1008715 (2020-06-20)
    Dysregulation of the Ras oncogene in development causes developmental disorders, "Rasopathies," whereas mutational activation or amplification of Ras in differentiated tissues causes cancer. Rabex-5 (also called RabGEF1) inhibits Ras by promoting Ras mono- and di-ubiquitination. We report here that Rabex-5-mediated
    A chromosomal region 7p11.2 transcript map: its development and application to the study of EGFR amplicons in glioblastoma.
    Eley GD
    Neuro-Oncology, 4(2), 86-94 (2002)

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