SRP6425
SERPIN A3 human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
AACT, ACT, GIG24, GIG25
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 46 kDa, observed mol wt 55-70 kDa (DTT-reduced. Protein migrates due to glycosylation. His 23 is the predicted N-terminal.)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 46 kDa, observed mol wt 55-70 kDa (DTT-reduced. Protein migrates due to glycosylation. His 23 is the predicted N-terminal.)
packaging
pkg of 10 μg
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... SERPINA3(12)
General description
Serpin A3 is also known as α-1-antichymotrypsin (ACT or AACT) gene with five exons and four introns spanning 12.8kb long, is mapped to human chromosome 14q32.13. The encoded protein is a 423 amino acid with the molecular weight of ~ 55-66kDa and it belongs to the family of serpin superfamily of protease inhibitors. Serpin A3 consists of three β sheets, eight α helices and an active site situated within a hypervariable reactive center loop (RCL).
Biochem/physiol Actions
Overexpression of SERPIN A3 leads to endometrial carcinoma (EC), Therefore targeting SERPIN A3 can be a potent therapeutic method for EC. In addition, elevated expression of SERPIN A3 expression has been observed in various types of carcinomas including breast carcinoma, hepatocellular carcinoma, prostate carcinoma and adenocarcinoma of the lung. SERPINA3 is associated with the pathogenesis of chronic obstructive pulmonary disease (COPD), Parkinson′s disease (PD), Alzheimer′s disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy. SERPIN A3 inhibits the activity of pancreatic chymotrypsin, leukocyte, cathepsin G, mast cell chymases, human glandular, kallikrein 2, kallikrein 3 (prostate specific antigen), pancreatic cationic elastase and an uncharacterized lung serum protease. SERPIN A3 can bind to DNA, this property is unique among the members of SERPIN family.
Physical form
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Down-regulated expression of SERPIN genes located on chromosome 18q21 in oral squamous cell carcinomas.
Shiiba M
Oncology Reports, 24(1), 241-249 (2010)
Crystal Baker et al.
Frontiers in bioscience : a journal and virtual library, 12, 2821-2835 (2007-05-09)
Recent advances in proteomic, transcriptomic and genomic technologies have revealed much about the ACT protein and gene. In this review, we summarize our current understanding of the structure and potential physiological roles of the ACT protein, catalogue the regulatory elements
SERPINA3 promotes endometrial cancer cells growth by regulating G2/M cell cycle checkpoint and apoptosis.
Yang GD
International Journal of Clinical and Experimental Pathology, 7(4), 1348-1358 (2014)
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