SRP6430
VEGF164 from mouse
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym(s):
VEGFA, VPF
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
mouse
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized
Mol wt:
calculated mol wt 19.3 kDa (no tag), observed mol wt 23-25 kDa (DTT-reduced. Protein migrates due to glycosylation. Ala 27 is the predicted N-terminal.)
biological source
mouse
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 19.3 kDa (no tag), observed mol wt 23-25 kDa (DTT-reduced. Protein migrates due to glycosylation. Ala 27 is the predicted N-terminal.)
packaging
pkg of 10 μg
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... VEGFA(22339)
General description
Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
Physical form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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