SRP6451
Fc gamma RIIIB/CD16b human
recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE)
Synonym(s):
CD16B, FCG3, FCG3B, FCGR3, FCGR3B, IGFR3
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About This Item
NACRES:
NA.32
UNSPSC Code:
51111800
Form:
lyophilized
Assay:
≥98% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 21.7 kDa, observed mol wt 38-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥98% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 21.7 kDa, observed mol wt 38-50 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.)
packaging
pkg of 10 and 50 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FCGR3B(2215)
General description
Cluster of differentiation 16 (CD16) is a low affinity type 1 transmembrane Fc receptor. It possesses two Ig-like extracellular domains. Cd16 has been identified as two isoforms, Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner. CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16B also known as FCGR3B and FCG3B, is expressed specifically by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils.
Biochem/physiol Actions
Cluster of differentiation 16B (CD16B) or Fcγ RIIIB (FcγRIIIb) is the low affinity receptor for the Fc region of immunoglobulin γ. It binds complexed or aggregated IgG and also to monomeric IgG. Contrary to CD16A, CD16B is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. CD16B may serve as a trap for immune complexes in the peripheral circulation, which does not activate neutrophils.
Physical form
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Find documentation for the products that you have recently purchased in the Document Library.
The Human Low Affinity Immunoglobulin G Fc Receptor III-A and III-B Genes MOLECULAR CHARACTERIZATION OF THE PROMOTER REGIONS.
Gessner J E, et al.
The Journal of Biological Chemistry, 270(3), 1350-1361 (1995)
Michael T L and Angus W T
Natural Killer Cells (2010)
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