SRP8056
Tim-3 (mouse): FC (human)
recombinant, expressed in CHO cells, ≥98% (SDS-PAGE)
Synonym(s):
HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIM3, TIMD3
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
mouse
recombinant
expressed in CHO cells
Assay
≥98% (SDS-PAGE)
form
lyophilized
mol wt
monomer 45 kDa by calculation
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
impurities
<0.06 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Havcr2(171285)
General description
Tim-3 is a type I transmembrane protein. It contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Type 1 T-helper (Th1) and Type 1 CD8+ T (Tc1) cells. TIM-3 is also expressed on some dendritic cells. The gene encoding this protein is localized on mouse chromosome 11B1.1.
Biochem/physiol Actions
The T cell/transmembrane, immunoglobulin and mucin (TIM) family plays a crucial role in regulating immune responses, such as allergy, asthma, transplant tolerance, autoimmunity and also has a control over the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3 generates an inhibitory signal resulting in apoptosis of Type 1 T-helper (Th1) and Type 1 CD8+ T (Tc1) cells. It can also mediate phagocytosis of apoptotic cells and cross-presentation of antigen. It functions to inhibit aggressive Th1-mediated auto- and allo-immune responses. Tim-3 pathway blockade by administration of Tim-3: Fc fusion protein accelerates diabetes in non-obese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade.
Physical form
Lyophilized from 0.2 μm-filtered solution in PBS.
Preparation Note
Reconstitute at 100 μg/mL in sterile PBS.
Other Notes
The extracellular domain of mouse Tim-3 (aa 21-189) is fused to the N-terminus of the Fc region of human IgG1.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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T and B cell hyperactivity and autoimmunity associated with niche-specific defects in apoptotic body clearance in TIM-4-deficient mice
Roselyn R
Proceedings of the National Academy of Sciences of the USA, 107.19, 8706-8711 (2010)
TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity
Gordon J. Freeman
Immunological Reviews, 235(1), 172-189 (2010)
TIM Proteins and Immunity
Lawrence P. Kane
Journal of Immunology, 184(6), 2743-2749 (2010)
TIM-3 is Expressed on Activated Human CD4+ T Cells and Regulates Th1 and Th17 Cytokines
William D. Hastings
European Journal of Immunology, 39(9), 2492-2501 (2009)
TIM-1 and TIM-3 Proteins in Immune Regulation
Ee Wern Su
Cytokine, 44(1), 9-13 (2008)
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