T1077
Tissue Inhibitor of Metalloproteinase-2 human
recombinant, expressed in CHO cells, >95% (SDS-PAGE), lyophilized powder
Synonym(s):
TIMP-2
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About This Item
Form:
lyophilized powder
Assay:
>95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in CHO cells
Mol wt:
apparent mol wt ~20 kDa
biological source
human
Quality Level
recombinant
expressed in CHO cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
mol wt
apparent mol wt ~20 kDa
technique(s)
activity assay: suitable, inhibition assay: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TIMP2(7077)
General description
Tissue inhibitor of metalloproteinase-2 (TIMP-2) is an extracellular inhibitor of matrix metalloproteinases (MMPs). There are six conserved cysteine residues forming disulfide loops in its amino- and carboxy-terminal domains. The gene encoding this protein is localized on human chromosome 17q25.
Biochem/physiol Actions
Tissue inhibitor of metalloproteinase-2 (TIMP-2), like other protease inhibitors, functions as a key modulator of extracellular matrix degradation during tissue development and remodeling. TIMP-2 can also act through a matrix metalloproteinase (MMP)-independent mechanism inhibiting endothelial cell proliferation in vitro. It demonstrates anti-angiogenic activities in vivo. TIMP-2 is a target gene of the microRNA-miR-22.
TIMP-2 has a greater binding efficiency to MMP-2 than the other MMPs. Although TIMP-2 is an inhibitor of MMP-2, it is also required at low concentrations for the activation of MMP-2.
Physical form
Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, and 150 mM sodium chloride, pH 7.5.
Analysis Note
The biological activity is measured by the ability to inhibit human MMP-2 hydrolysis of a fluorogenic MMP substrate.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Genetics of Moyamoya disease.
Roder C
Journal of Human Genetics, 55(11), 711-716 (2010)
Matrix MetalloProteinases (MMPs) andTissue Inhibitors of MetalloProteinases (TIMPs): positive and negative regulators intumor cell adhesion
Dimitra B
Seminars in Cancer Biology, 20(3), 161-168 (2010)
Growth-stimulatory activity of TIMP-2 is mediated through c-Src activation followed by activation of FAK, PI3-kinase/AKT, and ERK1/2 independent of MMP inhibition in lung adenocarcinoma cells.
Kim Hle
Oncotarget, 6(40), 42905-42922 (2015)
Promotion of astrocytoma cell invasion by micro RNA-22 targeting of tissue inhibitor of matrix metalloproteinase-2.
Ohnishi YI
Journal of Neurosurgery. Spine, 26(3), 396-403 (2017)
W G Stetler-Stevenson et al.
The Journal of biological chemistry, 265(23), 13933-13938 (1990-08-15)
Human tissue inhibitor of metalloproteinase-2 (TIMP-2) was cloned and sequenced from an A2058 human melanoma cell cDNA library. When the sequence was compared with that of human TIMP-1 at both the nucleotide and deduced amino acid levels, the homology appeared
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1077-10UG | 04061833226759 |
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