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Merck
CN

T2827

Sigma-Aldrich

Anti-dTAF80 antibody, Mouse monoclonal

clone 3D10, purified from hybridoma cell culture

Synonym(s):

Anti-TAF5, Anti-TAF80/85, Anti-TAFII80, Anti-dmTAF5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

3D10, monoclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

Drosophila melanogaster

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

indirect ELISA: suitable
western blot: 8-16 μg/mL using nuclear extracts of D.Mel cells.

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Drosophila melanogaster ... Taf5(47900)

General description

Monoclonal Anti-dTAF80 (mouse IgG2b isotype) is derived from the hybridoma 3D10 produced by the fusion of mouse myeloma cells and splenocytes from mice. Drosophila tightly associated factor 80 (dTAF80), also known as tightly associated factor 5 (TAF5) consists of numerous copies ofβ−transducin (WD40) repeat.

Immunogen

Drosophila TFIID complex.

Application

Anti-dTAF80 antibody, mouse monoclonal may be used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Drosophila tightly associated factor 80 (dTAF80) recognizes and interacts with a higher-order complex containing TATA box-binding protein (TBP), delta N250, 110 and 60.
Monoclonal Anti-dTAF80 reacts specifically with Drosophila dTAF80

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples shouldbe discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
018K4772

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Molecular cloning of a novel 120-kDa TBP-interacting protein
Yogosawa S, et al.
Biochemical and Biophysical Research Communications, 229(2), 612-617 (1996)
The dTAF II 80 subunit of Drosophila TFIID contains beta-transducin repeats
Dynlacht B D, et al.
Nature, 363(6425), 176-179 (1993)

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