T3399
Thrombin from bovine plasma
lyophilized powder, 50-150 NIH units/mg protein (biuret)
Synonym(s):
Factor IIa
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-648-7
MDL number:
form
lyophilized powder
specific activity
50-150 NIH units/mg protein (biuret)
composition
Protein, ~50%
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... F2(280685)
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Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Physical form
Lyophilized powder containing sodium chloride and Tris-HCl
Preparation Note
Prepared using rabbit brain thromboplastin for conversion to the active form.
Analysis Note
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Inhibitory effect of apixaban compared with rivaroxaban and dabigatran on thrombin generation assay.
Pancras C Wong et al.
Hospital practice (1995), 41(1), 19-25 (2013-03-08)
The effect of the oral direct activated factor X (factor Xa) inhibitor apixaban on tissue factor-induced thrombin generation in human plasma was investigated in vitro using the calibrated automated thrombogram (CAT) method and compared with the oral direct factor Xa
Dabigatran: a nephrological way out.
Patricio A Pazmiño
The American journal of medicine, 126(4), e21-e21 (2013-03-20)
Ildiko M Kovach et al.
Biochemistry, 52(14), 2472-2481 (2013-03-23)
Thrombin is the pivotal serine protease enzyme in the blood cascade system and thus a target of drug design for control of its activity. The most efficient nonphysiologic inhibitor of thrombin is hirudin, a naturally occurring small protein. Hirudin and
Response.
Sarah T Garber et al.
Journal of neurosurgery, 118(2), 483-483 (2013-03-16)
Christopher S King et al.
Chest, 143(4), 1106-1116 (2013-04-03)
Thromboembolic diseases are common. Heparins and the vitamin K antagonists have been the mainstay of therapy for > 60 years, but both classes of agents have limitations. The "ideal" anticoagulant should be as effective and safe as heparin and vitamin
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