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Merck
CN

T4000

Tetranitroblue tetrazolium chloride

Synonym(s):

3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride], TNBT, Tetranitrotetrazolium blue chloride

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About This Item

Empirical Formula (Hill Notation):
C40H28Cl2N12O10
CAS Number:
1184-43-6
Molecular Weight:
907.63
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24900185
EC Number:
214-665-1
Beilstein/REAXYS Number:
3897475
MDL number:
MFCD00036338

Key Documents

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InChI key

VCESGVLABVSDRO-UHFFFAOYSA-L

InChI

1S/C40H28N12O10.2ClH/c1-61-37-23-27(7-21-35(37)47-43-39(25-3-9-31(10-4-25)49(53)54)41-45(47)29-13-17-33(18-14-29)51(57)58)28-8-22-36(38(24-28)62-2)48-44-40(26-5-11-32(12-6-26)50(55)56)42-46(48)30-15-19-34(20-16-30)52(59)60;;/h3-24H,1-2H3;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccc(cc4)[N+]([O-])=O)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccc(cc8)[N+]([O-])=O

assay

≥90%

form

powder

mp

~170 °C (dec.)

solubility

water: 1 mg/mL, clear to hazy, yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
027K5307
07614TE
01007EH
017K5314
116K1491

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C L Jordan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(1), 239-247 (1989-01-01)
In the previous study (Jordan et al., 1989), we demonstrated that androgen treatment of juvenile male rats inhibits the elimination of synapses in the levator ani (LA) muscle. In the present study, we asked whether synapse elimination would occur once
C J van Noorden et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(12), 1401-1404 (1987-12-01)
We studied the effect of section thickness on the reaction rate of glucose-6-phosphate dehydrogenase (G6PD) activity in unfixed sections of rat liver by use of continuous monitoring by microdensitometry of the reaction product as it formed in the section during
W M Frederiks et al.
The Histochemical journal, 25(1), 86-91 (1993-01-01)
A quantitative histochemical procedure was developed for the demonstration of purine nucleoside phosphorylase in rat liver using unfixed cryostat sections and the auxiliary enzyme xanthine oxidase. The optimum incubation medium contained 18% (w/v) poly(vinyl alcohol), 100 mM phosphate buffer, pH
H Schmid
Basic and applied histochemistry, 28(3), 221-231 (1984-01-01)
A simple method for microphotometric evaluation of cryostat sections from human renal tissue routinely stained for succinate dehydrogenase activity by means of tetranitro-blue tetrazolium chloride is described and tested for validity. Manual absorbance measurement within single nephron segments from the
M S Letinsky
Stain technology, 58(1), 21-27 (1983-01-01)
Nitroblue tetrazolium (NBT) has been used to stain motor nerve terminals and unmyelinated axons in vertebrate skeletal muscle, but undesirable background connective tissue coloration resulted. This procedure was improved by separation of the tetrazolium salt's binding from its subsequent reduction.
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