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T4323

Tris-Borate-EDTA buffer

Name: Tris-Borate-EDTA buffer
Brand: SIGMA

BioReagent, 10× concentrate, suitable for electrophoresis

Synonym(s):

TBE buffer

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About This Item

UNSPSC Code:

12161700

MDL number:

MFCD00236358

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Properties

grade

Molecular Biology

sterility

sterile; 0.2 μm filtered

product line

BioReagent

impurities

DNase, RNase, Protease, none detected, bioburden, tested, endotoxin, tested, ≤5 ppm heavy metals (as Pb)

suitability

suitable for electrophoresis

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

Description

Application

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis.TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Disclaimer

TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.

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pictograms

GHS08

signalword

Danger

hcodes

H360FD

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

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The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis.

Josep Balart et al.

Radiation oncology (London, England), 6, 6-6 (2011-01-18)

Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms

A method for nanofluidic device prototyping using elastomeric collapse.

Seung-min Park et al.

Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15549-15554 (2009-09-01)

Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques