T4528
Thioglucosidase from Sinapis alba (white mustard) seed
≥100 units/g solid
Synonym(s):
Glucosinolase, Myrosinase, Thioglucoside glucohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/g solid
Biological source:
plant seeds (Sinapis alba)
biological source
plant seeds (Sinapis alba)
form
solid
specific activity
≥100 units/g solid
storage temp.
−20°C
Quality Level
Related Categories
General description
Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.
Application
Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples.
Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.
Biochem/physiol Actions
Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.
Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.
Other Notes
One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Fernando B Luciano et al.
International journal of food microbiology, 145(1), 69-76 (2010-12-15)
Work examines the origin of bactericidal activity in mustard flour and explores the relative contribution from starter cultures, E. coli O157:H7 itself and other sources. Bacteria can degrade naturally occurring glucosinolates in mustard and form isothiocyanates with antimicrobial activity. In
Palatability of Thlaspi caerulescens for snails: influence of zinc and glucosinolates.
Noret, et al.
The New phytologist, 165, 763-771 (2020)
Bioavailability of Isothiocyanates From Broccoli Sprouts in Protein, Lipid, and Fiber Gels
Oliviero T, et al.
Molecular Nutrition And Food Research, 62(18), 1700837-1700837 (2018)
Hanbin Dan et al.
Plant molecular biology, 63(2), 221-235 (2006-10-26)
Sulphate is a major macronutrient required for the synthesis of the sulphur (S)-containing amino acid cysteine and thus is critical for cellular metabolism, growth and development and response to various abiotic and biotic stresses. A recent genome-wide expression study suggested
Chemical defence and toxins of plants
Yamane H, et al.
Journal of Hospital Infection (2010)
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