Skip to Content
Merck
CN

T5569

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Synonym(s):

TMB substrate for ELISA (Super Slow)

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA, peroxidase substrate

form

liquid

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 60% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Super Slow, for ELISA has been used as a substrate for enzyme linked immunosorbent assay (ELISA).

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is an electrochemical substrate for low level detection of horse radish peroxidase (HRP). It is a highly sensitive and dual function substrate, since it produces both soluble and insoluble reaction products. It has rapid reaction rate and it is suitable for on-line kinetic analysis. It does not possess carcinogenic properties.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000523970
0000523970
0000493648
0000493648
0000490842

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Dimitris Missirlis et al.
Biophysical journal, 119(12), 2558-2572 (2020-11-21)
The mechanics of fibronectin-rich extracellular matrix regulate cell physiology in a number of diseases, prompting efforts to elucidate cell mechanosensing mechanisms at the molecular and cellular scale. Here, the use of fibronectin-functionalized silicone elastomers that exhibit considerable frequency dependence in
Anil G Cashikar et al.
Journal of lipid research, 64(4), 100350-100350 (2023-02-28)
Neuroinflammation, a major hallmark of Alzheimer's disease and several other neurological and psychiatric disorders, is often associated with dysregulated cholesterol metabolism. Relative to homeostatic microglia, activated microglia express higher levels of Ch25h, an enzyme that hydroxylates cholesterol to produce 25-hydroxycholesterol
Roberto Lande et al.
Antibodies (Basel, Switzerland), 9(2) (2020-05-15)
Human cathelicidin LL37 is a cationic antimicrobial peptide active against bacteria and viruses and exerting immune modulatory functions. LL37 can be also a target of autoreactive B- and T-lymphocytes in autoimmune settings. Irreversible post-translational modifications, such as citrullination and carbamylation
ApoE4 accelerates early seeding of amyloid pathology
Liu CC, et al.
Neuron, 96(5), 1024-1032 (2017)
Pan-CC chemokine neutralization restricts splenocyte egress and reduces inflammation in a model of arthritis
Buatois V, et al.
Journal of Immunology, 185(4), 2544-2554 (2010)
View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

Related Content

Data Sheet - T5569

Instructions

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service