T5778
Anti-Rat IgG (whole molecule)−TRITC antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
TRITC conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:160 using rat spleen cells or human peripheral blood lymphocytes
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsils
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Immunoglobulin G (IgG) is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.
Application
Conjugation of TRITC to Anti-Rat IgG (whole molecule) antibody enables its use in immunofluorescent techniques.
Biochem/physiol Actions
Immunoglobulin Gs (IgGs) are glycoprotein antibodies that modulate several immune responses. It plays a major role in providing immunological defense against infection to the infant from the mother via the placenta. IgG activates the complement system and regulates the antibody-dependent cell-mediated cytotoxicity (ADCC).
The antiserum is determined to be immunospecific for rat IgG.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Note: Store product protected from light.
Note: Store product protected from light.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Placental transfer of IgG subclasses in a Japanese population.
Hashira S, et al.
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