T7201
Thrombin from bovine plasma
lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
Synonym(s):
Factor IIa
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥2,000 NIH units/mg protein (E1%/280 = 19.5)
form
lyophilized powder
specific activity
≥2,000 NIH units/mg protein (E1%/280 = 19.5)
mol wt
heavy chain ~31 kDa, light chain ~6 kDa
storage temp.
−20°C
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General description
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin consists of A-chain and a catalytic B-chain with the catalytic pocket comprising of His57, Asp102, Ser195 and Ser214. It has a fibrinogen recognition site (FRS) and a heparin binding site (HBS) in the C-terminus. The main function of thrombin is cleavage of fibrinogen to fibrin. It coordinates the coagulation cascade and binds to diverse substrates. It coordinates with protease-activated receptors (PARs) to accomplish the physiological functions. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contribute to the progression of Parkinson′s disease. Altered thrombin levels modulates coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin.
Application
Thrombin from bovine plasma has been used in the cleavage of the maltose binding protein tag in mini-calpain-5 fusion protein and in thrombin/hirudin spiking experiments in serum samples.
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess a novel antiplatelet therapy in acute coronary syndromes.
Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
Physical form
Lyophilized from saline sodium citrate buffer, pH 6.5
Preparation Note
Traditional thrombin products are activated with bovine brain, whereas T7201 is activated with bovine lung and does not contain any bovine brain products.
Analysis Note
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Other Notes
Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Thrombin-mediated degradation of parathyroid hormone in serum tubes
Laulu SL, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 437, 191-196 (2014)
Small-angle X-ray scattering of calpain-5 reveals a highly open conformation among calpains
Gakhar L, et al.
Journal of Structural Biology, 196(3), 309-318 (2016)
Karin A L Müller et al.
Current vascular pharmacology, 10(5), 606-618 (2012-06-26)
Acute coronary syndromes (ACS) are triggered by enhanced platelet activation and aggregation. Hence, a cornerstone of successful secondary prevention in ACS is an effective platelet inhibition. Additionally, coronary interventions (PCI) lead to even increased artherothrombotic risks, another challenge in preventing
Directing thrombin
Lane DA, et al.
Blood, 106(8), 2605-2612 (2005)
Thrombin generation correlates with disease duration in multiple sclerosis (MS): Novel insights into the MS-associated prothrombotic state
Parsons MEM, et al.
Multiple Sclerosis Journal, 3(4), 84-84 (2017)
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